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https://www.sciencedirect.com/science/article/pii/S0076687976440120
Covalent Coupling Methods for Inorganic Support Materials By HOWARD H. WEETALL Choosing the Carrier Inorganic support materials have been shown to be excellent carriers for immobilized enzymes. 1-s The charaoteristics of these inorganic support materials are extremely important and play an definitive role in the choice of the proper carrier for the enzyme to be immobilized.Cited by: 475
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6272024/
Sep 09, 2014 · Among all these methods, covalent immobilization generally ensures the highest strength of the bonding between support and enzyme, minimizing leakage issues. Moreover, covalent attachment does not usually interfere with reagents/products mass transfer, and allows the highest enhancement of operational stability (especially towards heat, pH, organic solvents, and also …Cited by: 211
https://www.ncbi.nlm.nih.gov/pubmed/15183
1. Methods Enzymol. 1976;44:134-48. Covalent coupling methods for inorganic support materials. Weetall HH. PMID: 15183 [PubMed - indexed for MEDLINE]Cited by: 475
https://europepmc.org/articles/PMC6272024
The inorganic support needs, however, to be firstly functionalized with aromatic amine functions. This can be accomplished for instance by nitration and reduction of phenyltrialkoxysilane-treated supports (compare § 4.2), or by alkaline reaction between epoxy-activated support and p-phenylene-diamine . A 1:1 molar ratio between diamine and ...
https://journals.sagepub.com/doi/abs/10.1177/0885328204041443
Apr 01, 2004 · Results of a novel method of covalent bonding of an antibiotic (gentamicin) to silica bead supports are shown. Gentamicin was immobilized to four types of matrix: silica gel and porous glass beads activated by either silanization (APTES) or by adhesively bound keratin (with immobilization yield ranging from 36.5 to 91%).Cited by: 8
https://www.thermofisher.com/us/en/home/life-science/cell-analysis/qdots-microspheres-nanospheres/idc-surfactant-free-latex-beads/latex-bead-protein-coupling-protocols/covalent-protein-coupling-protocol.html
Covalent Protein Coupling Protocol › Described below is a method for the covalent attachment of protein to CML or Alipathic Amine IDC UltraClean latex beads. Coupling of the primary amino groups to carboxyl groups using a water soluble carbodiimide (WSC) is a common procedure.
https://link.springer.com/article/10.1007/BF02916421
Jun 01, 1993 · Today silane coupling is one of the commonly used techniques in the arsenal of the biochemist for the binding of material of all sorts to inorganic surfaces. Inorganic materials come in a variety of shapes, sizes, and characteristics. Today silane coupling is one of the most used coupling methods for the preparation of biosensing devices.Cited by: 362
https://link.springer.com/protocol/10.1385/0-89603-386-4:331
Immobilization by covalent methods must be carried out in chemical conditions that are compatible with the stability properties of the proteins. Generally, this involves two steps, first, activation of the support and second, coupling of the enzyme to the activated support.Cited by: 6
https://www.researchgate.net/publication/10721392_Covalent_Coupling_Method_for_Lipase_Immobilization_on_Controlled_Pore_Silica_in_the_Presence_of_Nonenzymatic_Proteins
Covalent Coupling Method for Lipase Immobilization on Controlled Pore Silica in the Presence of Nonenzymatic Proteins. Candida rugosa lipase was covalently immobilized on silanized controlled pore silica previously activated with glutaraldehyde in the presence of nonenzymatic proteins.
https://www.researchgate.net/publication/264773010_Microwave-assisted_covalent_immobilization_of_enzymes_on_inorganic_surfaces
Microwave-assisted covalent immobilization of enzymes on inorganic surfaces. Enzymes on carriers can be easily recycled or used in fixed bed reactors. The immobilization often results in an improved stability. Depending on the support used and the method of coupling this is a time consuming process.
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