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https://www.liebertpub.com/doi/10.1089/cell.2009.0106
Human embryonic stem cells (hESCs) are usually maintained in an undifferentiated state by coculture with mitomycin C-treated mouse embryonic fibroblasts (MEFs) as feeder cells in the presence of animal sera such as fetal bovine serum (FBS).Cited by: 16
https://www.researchgate.net/publication/45602824_Optimization_of_Culture_Conditions_to_Support_Undifferentiated_Growth_of_Human_Embryonic_Stem_Cells
Culture conditions that support the growth of undifferentiated human embryonic stem cells (hESCs) have already been established using primary human amnion epithelial cells (hAECs) as an ...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3348118/
Nowadays, most stem cell culture use defined serum-free media containing various additive or growth factors. For example, one of the major soluble factors added to culture medium for regulating stem cell self-renewal is bFGF. This growth factor supplements the medium used to culture undifferentiated hESC,...Cited by: 61
https://www.liebertpub.com/doi/10.1089/clo.2008.0047
Mouse and human embryonic stem (ES) cells are usually maintained in an undifferentiated state by coculture with mouse embryonic fibroblasts (MEFs) as feeder cells. In the case of mouse ES cells, addition of leukemia inhibitory factor (LIF) to culture media is also necessary to prevent cell differentiation.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3840968/
Culture conditions for human embryonic stem cells. In the first report of hESC derivation in 1998 , mouse embryonic fibroblast (MEF) cells were used as feeder cells to support the undifferentiated proliferation of hESCs, and leukemia inhibitory factor (LIF) and fetal bovine serum (FBS) were used as major components of the culture medium. However, the bovine serum generally used in cell cultures is a …Cited by: 9
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4503840/
The effect of rock inhibitor (Y-27632) in gelatin coat based culture on buffalo embryonic stem cells. The effect of Y-27632 supplementation of ES cell medium supplemented with FGF-2 and LIF on gelatin coated dishes on the mean area of ES cells and expression of pluripotency genes are summarized in …Cited by: 1
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