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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2845543/
The chemical synthesis and semisynthesis of proteins harbor the potential to overcome many of the disadvantages of current protein production methods (19, 29, 78). In particular, chemical synthesis using established solid-phase techniques are rapid to effect, easily automated, and facilitate purification.Cited by: 354
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0001164
BackgroundThere is growing interest in the attachment of proteins to solid supports for the development of supported catalysts, affinity matrices, and micro devices as well as for the development of planar and bead based protein arrays for multiplexed assays of protein concentration, interactions, and activity. A critical requirement for these applications is the generation of a stable linkage ...
https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-biology-learning-center/protein-biology-resource-library/pierce-protein-methods/covalent-immobilization-affinity-ligands.html
Small scale affinity purification using an antibody immobilized to a solid support. Chromatography has three main components: the mobile phase or solvent containing proteins, the stationary or solid phase also called the medium or resin (which may be agarose …
https://en.wikipedia.org/wiki/Protein_array
A protein microarray (or protein chip) is a high-throughput method used to track the interactions and activities of proteins, and to determine their function, and determining function on a large scale. Its main advantage lies in the fact that large numbers of proteins can be tracked in parallel. The chip consists of a support surface such as a glass slide, nitrocellulose membrane, bead, or ...
https://en.wikipedia.org/wiki/Solid-phase_synthesis
In chemistry, solid-phase synthesis is a method in which molecules are covalently bound on a solid support material and synthesised step-by-step in a single reaction vessel utilising selective protecting group chemistry. Benefits compared with normal synthesis in …
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3473064/
When monomers and aggregates coexist in the sample, it is difficult to distinguish the residual structure in the monomer from the aggregate. To address this problem, we have developed a solid-support EPR approach , in which the protein is tethered on a solid support to prevent protein aggregation. This approach allows the use of high protein ...Cited by: 5
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