Ultralink Biosupport Coupling

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UltraLink™ Biosupport - Thermo Fisher Scientific

    https://www.thermofisher.com/order/catalog/product/53110
    Thermo Scientific UltraLink Biosupport is a durable, azlactone-activated, beaded-polyacrylamide resin to covalently immobilize proteins and other primary amine biomolecules for preparation of affinity purification columns.Features of UltraLink Biosupport: High coupling efficiency and capacityimmobil

INSTRUCTIONS UltraLink Biosupport Medium

    http://wolfson.huji.ac.il/purification/PDF/affinity/PIERCE_UltralinkBioSupport.pdf
    UltraLink® Biosupport Medium is ideal for low- and medium- pressure chromatography. The following graph illustrates the relationship between the differential pressure on the beads and the linear flow velocity of fluid through the column. Figure 2. Pressure-flow relationship across UltraLink® …

INSTRUCTIONS UltraLink Biosupport

    https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0011317_UltraLnk_BioSup_UG.pdf
    Effect of time and temperature on protein coupling to UltraLink Biosupport. Goat anti-rabbit antibody was coupled using 10 mg beads and 1 mg protein/ml in 0.6 M sodium citrate, 0.1 M MOPS; pH 7.5. Coupled ligand was radiometrically determined.

Covalent Immobilization of Affinity Ligands Thermo ...

    https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-biology-learning-center/protein-biology-resource-library/pierce-protein-methods/covalent-immobilization-affinity-ligands.html
    The group is spontaneously reactive with amines; no additives or catalysts are needed to drive the coupling process. The Thermo Scientific UltraLink Biosupport is a durable, azlactone-activated, beaded-polyacrylamide resin to covalently immobilize proteins and other primary amine biomolecules for preparation of affinity purification columns.

Thermo Scientific UltraLink Iodoacetyl Resin and Micro ...

    https://www.fishersci.com/shop/products/ultralink-iodoacetyl-resin-micro-peptide-coupling-kit/p-4531512
    The bead structure and efficiently coupling chemistry of Iodoacetyl-Activated UltraLink Biosupport results in high protein binding capacity, high linear flow rates, low nonspecific binding and overall superior performance in affinity chromatography. UltraLink Resins are …

0514.5 Number Description - Thermo Fisher Scientific

    https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0011270_UltraLnk_Hydrazide_Resin_UG.pdf
    UltraLink Biosupport is a rigid, highly crosslinked, copolymeric and porous resin with high coupling capacity.2-8 The charge-free support has minimal nonspecific interactions with most sample types. The porosity, rigidity and durability of UltraLink Biosupport make it suitable for medium-pressure, fast-flow techniques involving large sample ...

Thermo Scientific UltraLink Biosupport:Life Sciences ...

    https://www.fishersci.com/shop/products/ultralink-biosupport/p-4531497
    Thermo Scientific™ UltraLink Biosupport is a durable polyacrylamide resin activated to enable the efficient and direct covalent immobilization of proteins and other biomolecules through their primary amines for use in affinity purification procedures.Description: UltraLink Biosupport

INSTRUCTIONS UltraLink Iodoacetyl Gel

    http://wolfson.huji.ac.il/purification/PDF/affinity/PIERCE_UltralinkIodoAcetyl.pdf
    scale bulk applications. UltraLink® Biosupport Medium is useful for medium- pressure techniques with rapid throughput. The support has a maximum linear flow rate of 3,000 cm/hour. Protocol for Coupling to UltraLink® Iodoacetyl Gel Note: The UltraLink® Iodoacetyl Gel must be stored in the dark.

Biosupport, UltraLink™ VWR

    https://uk.vwr.com/store/product/18535804/biosupport-ultralinktm
    The bead structure and efficient coupling chemistry of UltraLink™ Biosupport results in high protein binding capacity, high linear flow rates, low non specific binding and overall superior performance in affinity chromatography. UltraLink™ resins are ideal for medium pressure applications such as FPLC.Brand: Pierce

Thermo Scientific™ UltraLink™ Biosupport: Protein ...

    https://www.fishersci.co.uk/shop/products/ultralink-biosupport/p-4531497
    The bead structure and efficient coupling chemistry of UltraLink Biosupport results in high protein binding capacity, high linear flow rates, low nonspecific binding and overall superior performance in affinity chromatography. UltraLink Resins are ideal for medium pressure applications such as FPLC. Highlights:Description: UltraLink Biosupport

UltraLink™ Biosupport - Thermo Fisher Scientific

    https://www.thermofisher.com/order/catalog/product/53110
    Thermo Scientific UltraLink Biosupport is a durable, azlactone-activated, beaded-polyacrylamide resin to covalently immobilize proteins and other primary amine biomolecules for preparation of affinity purification columns.Features of UltraLink Biosupport: High coupling efficiency and capacityimmobil

INSTRUCTIONS UltraLink Biosupport Medium

    http://wolfson.huji.ac.il/purification/PDF/affinity/PIERCE_UltralinkBioSupport.pdf
    UltraLink® Biosupport Medium is ideal for low- and medium- pressure chromatography. The following graph illustrates the relationship between the differential pressure on the beads and the linear flow velocity of fluid through the column. Figure 2. Pressure-flow relationship across UltraLink® …

INSTRUCTIONS UltraLink Biosupport

    https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0011317_UltraLnk_BioSup_UG.pdf
    Effect of time and temperature on protein coupling to UltraLink Biosupport. Goat anti-rabbit antibody was coupled using 10 mg beads and 1 mg protein/ml in 0.6 M sodium citrate, 0.1 M MOPS; pH 7.5. Coupled ligand was radiometrically determined.

Covalent Immobilization of Affinity Ligands Thermo ...

    https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-biology-learning-center/protein-biology-resource-library/pierce-protein-methods/covalent-immobilization-affinity-ligands.html
    The group is spontaneously reactive with amines; no additives or catalysts are needed to drive the coupling process. The Thermo Scientific UltraLink Biosupport is a durable, azlactone-activated, beaded-polyacrylamide resin to covalently immobilize proteins and other primary amine biomolecules for preparation of affinity purification columns.

Thermo Scientific UltraLink Iodoacetyl Resin and Micro ...

    https://www.fishersci.com/shop/products/ultralink-iodoacetyl-resin-micro-peptide-coupling-kit/p-4531512
    The bead structure and efficiently coupling chemistry of Iodoacetyl-Activated UltraLink Biosupport results in high protein binding capacity, high linear flow rates, low nonspecific binding and overall superior performance in affinity chromatography. UltraLink Resins are …

0514.5 Number Description - Thermo Fisher Scientific

    https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0011270_UltraLnk_Hydrazide_Resin_UG.pdf
    UltraLink Biosupport is a rigid, highly crosslinked, copolymeric and porous resin with high coupling capacity.2-8 The charge-free support has minimal nonspecific interactions with most sample types. The porosity, rigidity and durability of UltraLink Biosupport make it suitable for medium-pressure, fast-flow techniques involving large sample ...

Thermo Scientific UltraLink Biosupport:Life Sciences ...

    https://www.fishersci.com/shop/products/ultralink-biosupport/p-4531497
    Thermo Scientific™ UltraLink Biosupport is a durable polyacrylamide resin activated to enable the efficient and direct covalent immobilization of proteins and other biomolecules through their primary amines for use in affinity purification procedures.Description: UltraLink Biosupport

INSTRUCTIONS UltraLink Iodoacetyl Gel

    http://wolfson.huji.ac.il/purification/PDF/affinity/PIERCE_UltralinkIodoAcetyl.pdf
    scale bulk applications. UltraLink® Biosupport Medium is useful for medium- pressure techniques with rapid throughput. The support has a maximum linear flow rate of 3,000 cm/hour. Protocol for Coupling to UltraLink® Iodoacetyl Gel Note: The UltraLink® Iodoacetyl Gel must be stored in the dark.

UltraLink™ Biosupport

    https://www.thermofisher.com/order/catalog/product/53110
    Thermo Scientific UltraLink Biosupport is a durable, azlactone-activated, beaded-polyacrylamide resin to covalently immobilize proteins and other primary amine biomolecules for preparation of affinity purification columns.

INSTRUCTIONS UltraLink Biosupport

    https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0011317_UltraLnk_BioSup_UG.pdf
    Proteins couple with high coupling capacity and efficiency (80%) to UltraLink Biosupport using a wide range of concentrations. For IgG, maximal coupling capacity was not reached at 11 mg IgG bound per ml of beads (resin) or 88 mg

INSTRUCTIONS UltraLink Biosupport Medium

    http://wolfson.huji.ac.il/purification/PDF/affinity/PIERCE_UltralinkBioSupport.pdf
    Pierce UltraLink® Biosupport Medium is a preactivated, ready-to-use bead designed for use in affinity chromatography. These beads are composed of a bis -acrylamide/azlactone copolymer that is slightly hydrophobic in its active form and highly

Thermo Scientific UltraLink Iodoacetyl Resin and Micro ...

    https://www.fishersci.com/shop/products/ultralink-iodoacetyl-resin-micro-peptide-coupling-kit/p-4531512
    The bead structure and efficiently coupling chemistry of Iodoacetyl-Activated UltraLink Biosupport results in high protein binding capacity, high linear flow rates, low nonspecific binding and overall superior performance in affinity chromatography. UltraLink Resins …

0514.5 Number Description - Thermo Fisher Scientific

    https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0011270_UltraLnk_Hydrazide_Resin_UG.pdf
    UltraLink Biosupport is a rigid, highly crosslinked, copolymeric and porous resin with high coupling capacity.2-8 The charge-free support has minimal nonspecific interactions with most sample types. The porosity, rigidity and durability of UltraLink Biosupport make it suitable for medium-pressure, fast-flow techniques involving large sample ...

Thermo Scientific UltraLink Biosupport:Life Sciences ...

    https://www.fishersci.com/shop/products/ultralink-biosupport/p-4531497
    Thermo Scientific™ UltraLink Biosupport is a durable polyacrylamide resin activated to enable the efficient and direct covalent immobilization of proteins and other biomolecules through their primary amines for use in affinity purification procedures.

Biosupport, UltraLink™ VWR

    https://uk.vwr.com/store/product/18535804/biosupport-ultralinktm
    The bead structure and efficient coupling chemistry of UltraLink™ Biosupport results in high protein binding capacity, high linear flow rates, low non specific binding and overall superior performance in affinity chromatography. UltraLink™ resins are ideal for medium pressure applications such as FPLC.

INSTRUCTIONS UltraLink Iodoacetyl Gel

    http://wolfson.huji.ac.il/purification/PDF/affinity/PIERCE_UltralinkIodoAcetyl.pdf
    scale bulk applications. UltraLink® Biosupport Medium is useful for medium- pressure techniques with rapid throughput. The support has a maximum linear flow rate of 3,000 cm/hour. Protocol for Coupling to UltraLink® Iodoacetyl Gel Note: The UltraLink® Iodoacetyl Gel must be stored in the dark.

Covalent Immobilization of Affinity Ligands Thermo ...

    https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-biology-learning-center/protein-biology-resource-library/pierce-protein-methods/covalent-immobilization-affinity-ligands.html
    Thermo Scientific SulfoLink Coupling Resin is beaded agarose with an iodoacetyl group at the end of a long spacer arm. Immobilization of sulfhydryls occurs through displacement of the iodine atom. UltraLink Iodoacetyl Resin uses the same chemistry but is built on the acrylamide-based UltraLink Biosupport …

INSTRUCTIONS UltraLink Iodoacetyl Gel

    https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0011278_UltraLnk_Iodoacetyl_Gel_UG.pdf
    The UltraLink Biosupport is a rigid, highly crosslinked, co-polymeric and porous resin with high coupling capacity. The charge-free support has minimal nonspecific interactions with most sample types.

Thermo Scientific™ UltraLink™ Biosupport: Protein ...

    https://www.fishersci.co.uk/shop/products/ultralink-biosupport/p-4531497
    UltraLink Biosupport is an alternative to CNBr- and NHS-activated agarose resins for immobilizing antibodies and other proteins to prepare affinity columns. The beaded resin is a hydrophilic copolymer of polyacrylamide and azlactone having a rigid polymeric structure with high surface area and pore volume.

Thermo Scientific UltraLink Biosupport:Life Sciences ...

    https://www.fishersci.com/shop/products/ultralink-biosupport/p-4531497
    Thermo Scientific™ UltraLink Biosupport is a durable polyacrylamide resin activated to enable the efficient and direct covalent immobilization of proteins and other biomolecules through their primary amines for use in affinity purification procedures.

Thermo Scientific CarboxyLink Coupling Resins and ...

    https://www.fishersci.com/shop/products/carboxylink-coupling-resins-immobilization-kits/p-4531505
    Thermo Scientific™ CarboxyLink™ Coupling Resins and Immobilization Kits ... CarboxyLink Resin is crosslinked beaded agarose (or acrylamide-based Thermo Scientific UltraLink™ Biosupport) that has been activated with diaminodipropylamine (DADPA) to contain long spacer arms, each of which have a primary amine at the end. ... CarboxyLink ...

Immunoprecipitation (IP)

    https://assets.fishersci.com/TFS-Assets/LSG/Application-Notes/TR0064-Immunoprecipitation-guide.pdf
    UltraLink® Biosupport, which have distinct advantages such as resistance to microbial attack, good chemical and pH stability and low non-specific binding. UltraLink Biosupport can also withstand pressures up to 1000 psi. There are some disadvantages compared to agarose - chiefly poor flow rates, mechanical stability and a tendency

0514.5 Number Description - Thermo Fisher Scientific

    https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0011270_UltraLnk_Hydrazide_Resin_UG.pdf
    UltraLink Biosupport is a rigid, highly crosslinked, copolymeric and porous resin with high coupling capacity.2-8 The charge-free support has minimal nonspecific interactions with most sample types. The porosity, rigidity and durability of UltraLink Biosupport make it suitable for medium-pressure, fast-flow techniques involving large sample ...

Thermo Scientific UltraLink Iodoacetyl Resin and Micro ...

    https://www.fishersci.com/shop/products/thermo-scientific-ultralink-iodoacetyl-resin-micro-peptide-coupling-kit-ultralink-iodoacetyl-micro-peptide-coupling-kit/pi20485
    Thermo Scientific UltraLink Iodoacetyl Resin and Micro Peptide Coupling Kit UltraLink Iodoacetyl Micro Peptide Coupling Kit Life Sciences:Protein Biology:Protein Extraction Thermo Scientific UltraLink Iodoacetyl Resin and Micro Peptide Coupling Fisher Scientific

Thermo Scientific UltraLink Biosupport Dry resin; 1.25g ...

    https://www.fishersci.com/shop/products/thermo-scientific-ultralink-biosupport-dry-resin-1-25g/PI53110
    UltraLink Biosupport is an alternative to CNBr- and NHS-activated agarose resins for immobilizing antibodies and other proteins to prepare affinity columns. The beaded resin is a hydrophilic copolymer of polyacrylamide and azlactone having a rigid polymeric structure with high surface area and pore volume.

INSTRUCTIONS UltraLink Iodoacetyl Gel

    https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0011278_UltraLnk_Iodoacetyl_Gel_UG.pdf
    The UltraLink Biosupport is a rigid, highly crosslinked, co-polymeric and porous resin with high coupling capacity. The charge-free support has minimal nonspecific interactions with most sample types.

Biosupport, UltraLink™ VWR

    https://uk.vwr.com/store/product/18535804/biosupport-ultralinktm
    The bead structure and efficient coupling chemistry of UltraLink™ Biosupport results in high protein binding capacity, high linear flow rates, low non specific binding and overall superior performance in affinity chromatography. UltraLink™ resins are ideal for medium pressure applications such as FPLC.

Thermo Scientific™ Pierce™ NeutrAvidin™ Resins UltraLink ...

    https://www.fishersci.co.uk/shop/products/pierce-neutravidin-resins/11829410
    NeutrAvidin Resins are prepared by covalently coupling NeutrAvidin Protein to the beaded agarose or UltraLink Biosupport using efficient and stable chemistries, resulting in NeutrAvidin Agarose and UltraLink Resins that are leach-resistant and stable at pH 2-11.

Thermo Scientific Pierce Streptavidin Resins :Life ...

    https://www.fishersci.com/shop/products/pierce-streptavidin-resins/p-4531324
    The products use a recombinant form of streptavidin with a mass of 53 kDa and a near-neutral pI. The resins are prepared by covalently coupling streptavidin to the porous agarose or UltraLink Biosupport using efficient and stable chemistries, resulting in Streptavidin Agarose and UltraLink Resins that are leach-resistant and stable at pH 2-11.

UltraLink Immobilized Carboxy Rockford, IL 61105

    http://wolfson.huji.ac.il/purification/PDF/affinity/PIERCE_UltralinkImmobCarboxy.pdf
    UltraLink™ Immobilized Carboxy is prepared on the azlactone-activated support, 3M Emphaze™ Biosupport Medium AB 1, using a reaction mechanism that results in a stable linkage. The support is hydrophilic charge-free, high capacity and highly

INSTRUCTIONS UltraLink Iodoacetyl Gel

    http://wolfson.huji.ac.il/purification/PDF/affinity/PIERCE_UltralinkIodoAcetyl.pdf
    scale bulk applications. UltraLink® Biosupport Medium is useful for medium- pressure techniques with rapid throughput. The support has a maximum linear flow rate of 3,000 cm/hour. Protocol for Coupling to UltraLink® Iodoacetyl Gel Note: The UltraLink® Iodoacetyl Gel must be stored in the dark.

Queuosine formation in eukaryotic tRNA occurs via a ...

    http://www.tara.tcd.ie/bitstream/handle/2262/31426/Queuosine+formation+in+eukaryotic+tRNA+occurs+via+a+mitochondria-localized.pdf?sequence=1
    and Qv1 protein bound to Ultralink Biosupport resin (Pierce). Coupling of TGT and Qv1 to resin was carried out overnight in 0.1 M MOPS, 0.6 M sodium citrate pH 7.5 at room temperature. The resin was quenched with 3 M ethanolamine, washed with 1 M NaCl and equilibrated with 10 mM Tris pH 7.4. Antisera were eluted with a 10

Thermo Scientific™ Pierce™ NeutrAvidin™ Resins UltraLink ...

    https://www.fishersci.co.uk/shop/products/pierce-neutravidin-resins/11829410
    NeutrAvidin Resins are prepared by covalently coupling NeutrAvidin Protein to the beaded agarose or UltraLink Biosupport using efficient and stable chemistries, resulting in NeutrAvidin Agarose and UltraLink Resins that are leach-resistant and stable at pH 2-11. The products are excellent choices for a variety of small- or large-scale affinity ...

Thermo Scientific™ CarboxyLink™ Coupling Resins and ...

    https://www.fishersci.co.uk/shop/products/carboxylink-coupling-resins-immobilization-kits/10509293
    Description These activated resins enable immobilization of carboxylate ligands for use in affinity protocols. CarboxyLink Resin is crosslinked beaded agarose (or acrylamide-based Thermo Scientific UltraLink™ Biosupport) that has been activated with diaminodipropylamine (DADPA) to contain long spacer arms, each of which have a primary amine at the end.

Thermo Scientific™ Pierce™ Streptavidin Resins UltraLink ...

    https://www.fishersci.pt/shop/products/pierce-streptavidin-resins/11889390
    The products use a recombinant form of streptavidin with a mass of 53 kDa and a near-neutral pI. The resins are prepared by covalently coupling streptavidin to the porous agarose or UltraLink Biosupport using efficient and stable chemistries, resulting in Streptavidin Agarose and UltraLink Resins that are leach-resistant and stable at pH 2-11.

Thermo Scientific™ Pierce™ Streptavidin Resins Fisher ...

    https://www.fishersci.ca/shop/products/pierce-streptavidin-resins/pi53116
    The products use a recombinant form of streptavidin with a mass of 53 kDa and a near-neutral pI. The resins are prepared by covalently coupling streptavidin to the porous agarose or UltraLink Biosupport using efficient and stable chemistries, resulting in Streptavidin Agarose and UltraLink Resins that are leach-resistant and stable at pH 2-11.

Biosupport, UltraLink™ VWR

    https://fi.vwr.com/store/product/18535804/biosupport-ultralinktm
    UltraLink™ Biosupport is an alternative to CNBr- and NHS-activated agarose resins for immobilising antibodies and other proteins to prepare affinity columns. The beaded resin is a hydrophilic copolymer of polyacrylamide and azlactone having a rigid polymeric structure with high surface area and pore volume.

Immunoprecipitation (IP)

    https://assets.fishersci.com/TFS-Assets/LSG/Application-Notes/TR0064-Immunoprecipitation-guide.pdf
    UltraLink® Biosupport, which have distinct advantages such as resistance to microbial attack, good chemical and pH stability and low non-specific binding. UltraLink Biosupport can also withstand pressures up to 1000 psi. There are some disadvantages compared to agarose - chiefly poor flow rates, mechanical stability and a tendency

Thermo Scientific™ UltraLink™ Iodoacetyl Resin and Micro ...

    https://www.fishersci.co.uk/shop/products/ultralink-iodoacetyl-resin-micro-peptide-coupling-kit-1/10771005
    The bead structure and efficiently coupling chemistry of Iodoacetyl-Activated UltraLink Biosupport results in high protein binding capacity, high linear flow rates, low nonspecific binding and overall superior performance in affinity chromatography. UltraLink Resins …

Protein A/G UltraLink Resin - Thermo Fisher Scientific

    https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0011265_Protein_AG_UltraLnk_Resin_UG.pdf
    The secreted Protein A/G contains four Fc-binding domains from Protein A and two from Protein G, making it a more universal tool to investigate and purify immunoglobulins. The Thermo Scientific Protein A/G UltraLink Resins use an azlactone-activated support that is hydrophilic, charge-free, high

Queuosine formation in eukaryotic tRNA occurs via a ...

    http://www.tara.tcd.ie/bitstream/handle/2262/31426/Queuosine+formation+in+eukaryotic+tRNA+occurs+via+a+mitochondria-localized.pdf?sequence=1
    and Qv1 protein bound to Ultralink Biosupport resin (Pierce). Coupling of TGT and Qv1 to resin was carried out overnight in 0.1 M MOPS, 0.6 M sodium citrate pH 7.5 at room temperature. The resin was quenched with 3 M ethanolamine, washed with 1 M NaCl and equilibrated with 10 mM Tris pH 7.4. Antisera were eluted with a 10

Protein Purification Affinity purification - Coupling

    https://www.fishersci.co.uk/webfiles/uk/web-docs/UKLS_0670.PDF
    Protein Purification Affinity purification - Coupling GE NHS HP SpinTrap Enrichment of target proteins. • Antibody or capturing molecule covalently immobilised via free NH 2 groups • Simple coupling, carried out near neutral pH • Fast and flexible protocol for the capture step, with elution conditions formatted for both electrophoresis ...

UltraLink - Lindab

    http://www.lindab.com/global/pro/Pages/ultralink-next.aspx
    The popular Ultralink has now become even more intelligent and easy to control. The new edition of Ultalink comes with bluetooth technology for wirelss and easy commissioning. Discover UltraLink ® Controller – a new volume flow regulator

Fisher Scientific

    https://www.fishersci.ca/shop/coupon.do?cid=PROD_3772169
    The products use a recombinant form of streptavidin with a mass of 53 kDa and a near-neutral pI. The resins are prepared by covalently coupling streptavidin to the porous agarose or UltraLink Biosupport using efficient and stable chemistries, resulting in Streptavidin Agarose and UltraLink Resins that are leach-resistant and stable at pH 2-11.

INSTRUCTIONS Pierce CDI-Trisacryl

    https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0011170_Pierce_CDI_Trisacryl_UG.pdf
    The coupling process occurs at basic pH (8.5-10). While having slower reaction with proteins than reductive amination (Thermo Scientific AminoLink Coupling Gel, Product No. 20281) or azlactone (Thermo Scientific UltraLink Biosupport, Product No. 53110), CDI resins are particularly adept at

Naturally occurring autoantibodies against α-synuclein ...

    https://www.sciencedirect.com/science/article/pii/S0969996118304017
    The packing material coupled with α-syn oligomers for affinity chromatography was prepared by mixing 10 mg coupling UltraLink Biosupport resin (Thermo Scientific, Waltham, MA, USA) with 100 μL (1 mg/mL) α-syn oligomers in coupling buffer (0.2 M sodium carbonate, 0.6 M sodium citrate, pH 10) with shaking for 1 h at RT.

Heat shock protein 90 catalyzes activation of the ...

    https://www.pnas.org/content/99/2/896
    Jan 22, 2002 · Purified corn trypsin inhibitor (CTI, Enzyme Research Laboratories) was coupled to Ultralink Biosupport medium (Pierce) according to the manufacturer's recommendations. In brief, 10 mg of CTI was dissolved in 6 ml of 0.1 M carbonate buffer (pH 9.0), and sodium citrate was added to a final concentration of 0.6 M.

1,2, Li Peng1, Robert M. Woods 1, Herren Wu 1, William F ...

    https://www.researchgate.net/profile/Li_Peng14/publication/275667366_Structural_Insights_Into_the_Neutralization_Properties_of_the_Fully_Human_Anti-Interferon_Monoclonal_Antibody_Sifalimumab/links/5570648a08aeffcab353bd54.pdf?inViewer=true&disableCoverPage=true&origin=publication_detail
    1 Structural Insights Into the Neutralization Properties of the Fully Human, Anti-Interferon Monoclonal Antibody Sifalimumab Vaheh Oganesyan 1,2, Li Peng1, Robert M. Woods 1, Herren Wu 1, William ...

Binding of Pigment Epithelium-derived Factor (PEDF) to ...

    http://www.jbc.org/content/274/44/31605.long
    A total of 5.5 ml of dialysate or of coupling buffer was transferred to a tube containing 0.25 g of dry Ultralink TM biosupport medium, and the manufacturer's instructions were followed. The coupling reaction was performed by incubation with gentle rotation at 25 °C for 3 h.

Elastase-coupled beads as a tool for characterizing ...

    https://www.physiology.org/doi/full/10.1152/japplphysiol.00026.2013
    Intratracheal elastase challenge of laboratory animals has long been established as a model for observing the physiological and morphological changes that result from alveolar destruction, the hall...

Mice infected with Schistosoma mansoni generate antibodies ...

    https://academic.oup.com/glycob/article/9/10/1029/567830
    Coupling was carried out at room temperature for 4 h with rotation followed by overnight rotation at 4°C. The final coupling density was ∼15 mg milk glycoproteins per ml of biosupport medium. Pooled sera from S.mansoni infected mice (1 ml) was mixed with 7 ml of PBS and applied to a 2 ml milk glycoproteins/UltraLink column. The column was ...

Journal of Cell Science

    https://jcs.biologists.org/content/115/8/1759
    This indicates that fa2 cells are not defective in the coupling of cell size to commitment and provides an opportunity to assess rates of cell growth after return to the light. Fig. 6A shows that 10 hours after a shift into the light, both populations of cells increased in mean volume; fa2 …

Quantification of proteins - Bar-or, David

    http://www.freepatentsonline.com/y2006/0199280.html
    Sep 07, 2006 · ULTRALINK® Iodoacetyl Gel comprises UltraLink® Biosupport that has been derivatized with 15-atom spacer arms, each of which ends in an iodoacetyl group. ULTRALINK® Biosupport is a charge-free, rigid, highly cross-linked, copolymeric and porous resin with high coupling capacity and minimal nonspecific interactions with most sample types.

Immunoglobulin G modulation of the melanocortin 4 receptor ...

    https://www.nature.com/articles/s41398-019-0422-9
    Melanocortin 4 receptor (MC4R) plays a key role in regulation of appetite activated by its main ligand α-melanocyte-stimulating hormone (α-MSH) in both central and peripheral targets. α-MSH ...

Type 1 Parathyroid Hormone Receptor (PTH1R) Nuclear ...

    https://academic.oup.com/endo/article/147/7/3326/2500890
    A PTH affinity column was generated using the UltraLink immobilization kit according to the manufacturer’s instructions (Pierce). Briefly, 50 μg PTH (1–84) was dissolved in carbonate coupling buffer and added to the UltraLink Biosupport medium. The gel-PTH mixture was incubated for 1 h at room temperature with mixing.

Intravenous Immunglobulin Binds Beta Amyloid and Modifies ...

    https://www.researchgate.net/profile/David_Finkelstein4/publication/236930719_Intravenous_Immunglobulin_Binds_Beta_Amyloid_and_Modifies_Its_Aggregation_Neurotoxicity_and_Microglial_Phagocytosis_In_Vitro/links/00b495237f454371da000000.pdf?origin=publication_detail
    Intravenous Immunglobulin Binds Beta Amyloid and Modifies Its Aggregation, Neurotoxicity and Microglial Phagocytosis In Vitro Susann Cattepoel1*, …

TRAFD1 (FLN29) Interacts with Plekhm1 and Regulates ...

    https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0127537
    Anti-Plekhm1 antibody was raised in rabbits against the N-terminal 497 amino acids of human Plekhm1 protein using a standard immunization protocol (Capralogics Inc., Hardwick, MA), and the antiserum was affinity purified against the immunizing protein using UltraLink Biosupport resin …

TRIP/NOPO E3 ubiquitin ligase promotes ubiquitylation of ...

    https://dev.biologists.org/content/141/6/1332
    For co-immunoprecipitations, lysates (500 μg) were incubated 2 hours with shaking at 4°C with 50 μl of Ultralink Biosupport resin (Thermo Scientific) coupled to anti-MYC (9E10) or anti-FLAG (M2) antibodies. Beads were washed three times with NDLB.

Electrospray Ionization–Ion Mobility Spectrometry ...

    https://pubs.acs.org/doi/full/10.1021/ac301527v
    Macroion mobility spectrometry was used to distinguish between a monoclonal antibody (clone M612165) that bound exclusively to monomeric prostate specific antigen and a different monoclonal antibody (clone M612166) that bound exclusively to a dimeric form of the antigen that only comprised 6.8% of the total protein. In the presence of excess antigen, the mobility spectrum of M612165 was ...

Intravenous Immunglobulin Binds Beta Amyloid - …

    https://mafiadoc.com/intravenous-immunglobulin-binds-beta-amyloid_5d0445f2097c4700648b4567.html
    Intravenous Immunglobulin Binds Beta Amyloid and Modifies Its Aggregation, Neurotoxicity and Microglial Phagocytosis In Vitro Susann Cattepoel1*, Alexander Schaub1, Miriam Ender1, Annette Gaida1, Alain Kropf1, Ursula Guggisberg1, Marc W. Nolte2, Louis Fabri3, Paul A. Adlard4, David I. Finkelstein4, Reinhard Bolli1, Sylvia M. Miescher1 1 CSL Behring AG, Bern, Switzerland, 2 CSL …



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